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After two days of dehydration in 30% sucrose in PBS, the brains were cut into slices of 60 m thickness by cryosectioning and stored in PBS

Selenium speciation in paired serum and cerebrospinal fluid samples

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The authors proposed a model, in which the early burst of ROS and NO initiates HR-PCD but at later stages of the defence response the SNO levels exceed a certain threshold and subsequently the AtRBOHD is inactivated by S-nitrosylation at Cys 890, which terminates the HR