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Colony forming unit (CFU) assay For colony formation assays, cells were plated at 100 cells per 9.6 cm 2

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378 On the basis of the increased activity of -gal in senescent cells, a new prodrug, SSK1, which can be specifically cleaved by lysosomal -gal into cytotoxic substances and induce the apoptosis of senescent cells, thereby eliminating senescent cells, was developed