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Oxidative stress and hypoxia-induced RPE cell death have long been considered to play crucial roles in the initiation and progression of AMD, and ferroptosis was identified as one of the main cell death mechanisms of oxidative stress (Henning et al., 2022

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Immunostaining The slices were blocked with PBS containing 2.5%, with PBS containing 2.5% bovine serum albumin (BSA

Conceptual schematic illustrating the attenuation of circadian rhythm amplitude and phase shifts in aging, with a particular emphasis on nocturnal melatonin decline as a central driver of sleep fragmentation, metabolic dysregulation, and reduced antioxidant capacity ( The figure highlights age-related changes in key physiological variables, including restactivity cycles, core body temperature, hormonal secretion (melatonin and cortisol), suprachiasmatic nucleus (SCN) neuronal activity, and metabolic indicators such as glucose regulation

Similarly, an original patient-derived glioblastoma cell line, KNS1451, obtained from Kyushu University Brain Tumor Bank, was cultured as previously reported 9