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Research by Sikiri and colleagues demonstrated that BPC-157 inhibited melanoma cell line growth, reduced lung metastases in mice, and counteracted tumor cachexia in colon adenocarcinoma models

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The supernatant was discarded carefully and the cells were resuspended in 3 ml of chilled (80 mM CaCl 2 and 50 mM MnCl 2 ) buffer and kept on ice for 30 min