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Reactions were performed in 50 mM sodium phosphate buffer pH 7.0 containing 0.1% Triton-X-100, 0.40 mM EDTA, 0.20 mM NADPH, 0.25 U glutathione reductase from Bakers yeast, 1 mM L-glutathione, and 100 M t -butyl hydroperoxide (all Sigma-Aldrich) in a total volume of 200 l

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They worked in pairs, and disagreements were resolved by the fifth researcher (RC)

doi: 10.1157/13054318 91 QuJLiYZhongWGaoPHuC

Lubrication and Cushioning: Hyaluronic acid is a naturally occurring substance found in joint fluid that helps lubricate and cushion the joints