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Expression and purification of recombinant methyltransferase proteins NSD1(SET) (amino acids 18532093, NCBI sequence: NC_000005.10), NSD2(SET) (amino acids 9581365, NCBI sequence: NC_000004.12), NSD3(SET) (amino acids 10211320, NCBI sequence: NC_000008.11), ASH1L(SET) (amino acids 19802564, NCBI sequence: NC_000001.11), SETD2(SET) (amino acids 13232564, NCBI sequence: NC_000003.12) and SUV39H1(SET) (amino acids 82412, NCBI sequence: NC_000023.11) were cloned into pGEX-6P-1

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The functional annotation analysis identified, as highly enriched cluster related to GTPase activity, cytoskeletal binding, protease inhibitor, catalytic activity, transporter activity, protein-tyrosine kinase activity, and SOD (Fig

However, most of the experimental tests have been conducted in animal models and in vitro experiments, and only a few clinical trials have been conducted

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