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Four strains were inoculated in 5 mL LB liquid medium and incubated overnight in a rotary shaker (150 rpm) at 28

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196,197,198 The phosphorylation of Akt is necessary for its full activation, allowing it to regulate a variety of downstream effector proteins involved in cell survival, proliferation, metabolism, and glucose transport

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Each milliliter contains 1.0 mg of cyanocobalamin, ensuring effective supplementation