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Global loss of desnutrin/ATGL gene function in mice resulted in increased weight gain and a shift in favor of carbohydrate over fat as a primary fuel source during fasting, indicating that in vivo desnutrin/ATGL likely also functions in adipose tissue lipolysis (43)

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The background staining (BOD) of each image was measured by calculating the optical density of the area of central vein

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Testing before treatment is not just a best practice it is the standard recommended by the NIH, the AAFP, and the NICE clinical guideline NG239 (2024)