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One-half of each brain was flash frozen in liquid nitrogen for subsequent HPLC analysis of GSH (discussed below)

For proliferation assays, cells (5 10 4 per well) were plated in six-well plates with medium replacement every 48 h, followed by hemocytometer counting

Furthermore, the dose of melatonin used is still a matter of study

Differential analysis of plasma concentrations of metabolites from different categories revealed an increase in many phosphathidylcholines, triglycerides, and sphingomyelines in the plasma from cKOt mice (Figures 3J and Supplemental Figure 2B)