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Moreover, MTX blocks tetrahydrofolate dependent steps in cell metabolism resulting in adenosine overproduction which inhibits TNF- expression in a monocytic cell line and monocytes release adenosine after treatment with MTX [32]

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Although neurons possess a full complement of glycolytic enzymes, their expression of 6-phosphofructo-2-kinase (PFK2) is extremely low, especially the Pfkfb3 isoform (Almeida et al., 2004)