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Description

Naunyn Schmiedebergs Arch Pharmacol 398(5):51295145

19,20 PhastGelTM Semi-dry Isoelectric focusing (IEF) for evaluation of antibody clonality To perform an IEF analysis, the samples of purified antibodies were applied to a gel (polyacrylamide) with electric current applied to create migration using the Pharmacia PhastSystemTM and PhastGelTM IEF Gradient 3-9 separation gels, PhastGelTM buffer strips and full-range rainbow molecular weight markers (GE Healthcare, Piscataway, NJ)