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DNA methylation Genomic DNA was isolated from the liver tissue or cultured cells using an AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germantown, MD) following the manufacturers instructions

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Individual differences in anxiety-like behaviors among genetically similar Sprague Dawley and Lewis male rats, living in the same environments and not previously exposed to experimental manipulations, have also been shown to predict lifespan and prefrontal cortical dendritic length [21, 22]

10.3390/pharmaceutics17020244 30 GuoY.GaoF.AhmedA.RafiqM.YuB.CongH.et al (2023)

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At rest, the average heart beats about 72 times per minute and 103,680 times per day