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From Dougs explanation, you can see that using the 4.1 volts keeps you off the top end and setting an ESC low-voltage cutoff above the traditional 3 volts per cell will keep you off the bottom end

Jungert et al., 2020) whereas 80% and 71% of the investigated Malaysian and Cambodian women of reproductive age, respectively were reported to be riboflavin deficient (Aljaadi et al., 2019

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The dialyzed proteins were concentrated using the 3 K centricon (Millipore, Sigma) and either proceeded fresh for the droplet formation assays or aliquoted and flash frozen in liquid nitrogen and stored at 80 C