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After collection of the ascitic fluid, it was centrifuged at 1200 rpm for 2 min at room temperature, to remove the cells avoiding cell lysis

Indeed, a significantly lower glutathione-S-transferase (GST) enzyme activity and significantly high MDA levels have been shown in the cumulus cells of poor responders compared to high responders (220)

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This is consistent with IGF-1's insulin-like activity at the IGF-1 receptor and at insulin receptors at higher concentrations

This indicated that the mice experienced smoother breathing, with a more effective increase in expiratory volume and an increase in respiratory depth during expiration

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