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Slides were dried for at least 20 hours at 4C and fluorescent images were obtained with Slidebook 4.2 Digital Microscopy Software driving an Olympus 1X81 inverted confocal microscope with a 60X oil immersion lens, NA 1.4 and resolution 0.280 Dendritic spine density was measured on primary and secondary dendrites at a distance of at least 150 from the soma

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Vitamin B12 itself is vital for energy production and maintaining healthy nerve cells, but its direct impact on weight loss needs to be clearly established

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The mix is swirled gently never shaken and stored refrigerated at 35.6-46.4 F (2-8 C)