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[1] [6] In healthy adults, vitamin B 12 deficiency is not common, mainly because body stores of the vitamin are substantial and turnover is slow, with relatively low dietary requirements

After the centrifuge, cells were resuspended in 20 mL proliferation medium containing Neural Stem Cell Basal Medium (SCM003, Merck Millipore), B27 without vitamin A (12587010, Thermo Fisher Scientific, Massachusetts, USA), GlutaMAX (35050061, Thermo Fisher Scientific), antibiotics (100 IU/mL penicillin and 100 g/mL streptomycin), 1 mg/mL epidermal growth factor (E9644, Sigma-Aldrich), and 1 mg/mL fibroblast growth factor 2 (100-18B, PeproTech, NJ, USA), and incubated at 37 C with 5% CO 2

To address this, a research team led by Wang et al

Cartron G, Dacheux L, Salles G, Solal-Celigny P, Bardos P, Colombat P

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