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Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark

Maintaining a healthy weight is one of the most important steps men can take to maintain or restore fertility

10.1002/art.20626 219 ParkJ.KwonO.AnH.-J.ParkK

This finding does not establish causality, but it is consistent with a model in which robust mitochondrial peptide signaling is a feature of successful aging rather than a peripheral correlate