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Thymosin beta-4 binds to monomeric G-actin with a 1:1 stoichiometry, forming a complex that prevents spontaneous polymerization of actin into filaments (F-actin)

Although the precise mechanisms that underlie the observed differences between the neutralizing titers elicited by membrane-anchored gH/gL and the ectodomain are not clear, we note that a similar phenomenon was observed when comparing mRNA delivery of membrane-anchored and secreted MERS Spike protein 63 , and that mRNA-based SARS-CoV-2 vaccines are based on full length rather than secreted spike proteins 63,64,65,66

Anti-doping labs may still detect traces using highly sensitive mass-spectrometry methods, but standard screening does not track these short-lived compounds

Weinberg SE, Sena LA, Chandel NS