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Later, we will meet in Aguas Calientes to board the Vistadome or Expedition train (depending on availability) for the return to Ollantaytambo , then continue by comfortable transportation to the city of Cusco

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For qPCR analysis, 5 L of immunoprecipitated DNA was used as a template in a 10 L total reaction volume using TaqMan Advanced PCR Master Mix (Thermo Fisher Scientific) and target-specific TaqMan probes, prepared in 384-well plates