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Unlike full-length growth hormone, AOD-9604 administration did not affect IGF-1 levels or glucose homeostasis

Structure 1995

Briefly, cells cultured on a Transwell membrane were washed with PBS containing 0.2 mM CaCl 2 and 2 mM MgCl 2 (PBS-C/M), followed by fixation with 4% paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA, USA) in PBS-C/M for 10 min at room temperature

Mechanism of Action MOTS-c: Functions as a metabolic activator

Second, as mentioned above, c-Met inhibitors which target the interaction between HGF and c-Met may lose efficacy owing to cell attachment (Wang et al., 2001), gene amplification of c-Met, DCP binding to c-Met (Suzuki et al., 2005), gene mutation in the c-Met activation loop (Okuma and Kondo, 2016) and crosstalk with other membrane receptors (Garcia-Vilas and Medina, 2018)

doi: 10.1016/j.jprot.2013.04.013 17 DewhirstF