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Infertility may also occur in individuals with insufficient B12

In the left you can see the B12 tucked into the structure, such that it is buried within the protein (right) The major cause of Paradoxical Vitamin B12 Deficiency appears to lack of functional vitamin B2, which may occur due to overt vitamin B2 deficiency in a person's diet, Hypothyrodism (Habbar etal, 2008), or due to lack of adequate intake of Iodine, Selenium and/or Molybdenum, which in turn leads to insufficient production of the two active forms of vitamin B2, namely FMN and FAD

Then a Cell Counting Kit-8 (CCK-8) (Sigma, USA) was used to assess cell viability in line with manufacturers instructions

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