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Bioavailability and daily requirement of vitamin B(12) in adult humans: an observational study of its colonic absorption and daily excretion as measured by [(13)C]-cyanocobalamin kinetics

Aplin JD, Myers JE, Timms K, Westwood M

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Lysates were diluted to final concentrations of 200 mM NaCl, 0.5% NP-40, and 0.05% Na-deoxycholate, and incubated with GFP-binding protein (GBP) beads for 2 h at 4 C