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Description

The model fit was impaired without lag time and not relevantly improved by a two-compartment model

For immunofluorescence, cryosections were dried for 2 h at RT, fixed in pure acetone for 10 min and blocked with PBS 10% FCS for 30 min at RT

12 , CD005225 (2012)

The TEV protease and the -mercaptoethanol were added in the pooled fraction with target protein to TEV protease ratio of 1:10 (w/w) and the final concentration of 10 mM, respectively, to cleave 6HFh8 and dialyzed against 1 PBS with 150 mM NaCl at 4 C overnight