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CVCL 0038) 54 were derived as published

The structures of ghrelin- and ibutamoren-bound GHSR share similar overall conformation with a root-mean-square deviation (RMSD) of the C atoms at 1.23

Subsequently, the muscle pieces were fixed overnight at 4C in 0.1 M PIPES buffer with 2 mM CaCl 2 , 3% PFA and 2.5% glutaraldehyde

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Western blot analysis quantified protein expression levels, utilizing total protein extracts as well as nuclear and cytoplasmic fractions