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After 72 h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution (25 L/well, at a final concentration of 5 mg/mL) was added to each well 4 h before the end of the culture

Wang L, Zhang T, Zheng Y, Li Y, Tang X, Chen Q, et al

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Neuroprotective effect of baicalein against MPTP neurotoxicity: Behavioral, biochemical and immunohistochemical profile

The slides were washed and then incubated with biotin-labeled goat anti-rabbit secondary antibody at 37 C for 30 min

More studies with larger sample sizes are needed in this area to make a general conclusion