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For transfection experiments, cells were seeded into six-well plates overnight and were transiently transfected using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturers instructions

a , Percentage of IL-2 producing cells (mean SEM) among unstimulated IFN- + mouse CD8 + T cells, or ex vivo-stimulated IFN- + mouse CD8 + T cells that were either left untreated (non-arrested), arrested in the cell cycle using RO-3306 (arrested), or released from RO-3306-induced arrest ( n =5 mice)

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