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Jardines, D

Glutaminase (GLS) generates glutamate via glutaminolysis to support anaplerosis but generates more ammonia ( Fig

Tumor tissues were excised from anesthetized mice and homogenized in HEPES-buffered saline containing 10% glycerol, 1 mM EDTA, 2 mM EGTA, 1 mM DTT, 5 mM Na 3 VO 4 , 5 mM NaF, 1 mM AEBSF, aprotinin (5 g/ml), and leupeptin (5 g/ml) using a Dounce homogenizer