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The protein was purified with Strep-affinity chromatography (for more details see supplementary figure 2), the tag was removed by TEV-cleavage and the protein was dialyzed into buffer W (100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8.0)

Abnormal transmethylation/transsulfuration metabolism and DNA hypomethylation among parents of children with autism

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Future work should focus on the degradation of glutathione using ChaC and PepD, and if this can be detected using out biosensor

and for vial-size principles generally, see Peptide Vial Sizes Explained