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Cell viability was calculated using the formula: Antioxidant Efficacy Peripheral blood mononuclear cells (PBMCs) were isolated from 10 mL of peripheral blood obtained from healthy volunteers after ethical approval and written informed consent (PUIECHR/PIMSR/00/081734/7212)

To understand the contribution of GCN2 to the broad rewiring of the transcriptome in Leu- or Arg-starved ES cells, we generated Eif2ak4 -deficient ES cells by CRISPR/Cas9 targeting (Fig S1B)