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The resistance of the patch pipette was 45 M, and the intracellular solution contained (mmol/L): 110 choline chloride, 2.5 KCl, 20 glucose, 1.3 NaH 2 PO 4 , 0.5 CaCl 2 , 7 MgCl 2 , 25 NaHCO 3 , and 1.3 Naascorbate

Most of the studies on heavy metal toxicity in plants indicate that NO reduces the ROS levels by enhancing the levels of antioxidative enzymes ( 2 .- ) ( Figure 1 Other studies on heavy metal toxicity suggested the role of nitric oxide in controlling the stomatal aperture ( Nitric oxide interacts with different biomolecules like phytohormones in response to heavy metal stress in plants

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On day 14, the MTT assay was performed