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Intramuscular (IM): This requires a longer needle to deliver the solution directly into the muscle tissue

224 It is estimated to affect roughly 14% of middle-aged women

To strengthen the assumption that only the cyclic version of G4CP2 is responsible for the observed phenotypes, we generated a G4CP2 variant (linear G4CP2), where a stop codon is inserted between the G4CP2 sequence and the Intein N element (Supplementary Figure S7A)

[DOI] [PMC free article] [PubMed] [Google Scholar] 230

doi: 10.19540/j.cnki.cjcmm.20221212.601 46 KongQZhuHGongWDengXLiuBDongJ

Four whole bodies of female flies were homogenised in 160 L of 80% methanol containing 10 M internal standards (methionine sulfone and 2-morpholinoethanesulfonic acid)