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The specificity of the reaction was demonstrated by competitive inhibition using recombinant STX7

Common injection sites include: Anterior, inferior, and posterior glenohumeral ligaments Middle glenohumeral ligament (MGHL) Posterior capsule (for posterior instability) Rotator interval region Long head of biceps anchor (if involved) By treating these areas, prolotherapy helps restore the biomechanical integrity of the joint capsule, thereby reducing: Pain Recurrent subluxation or dislocation Weakness Activity limitations Ideal Candidates for Prolotherapy Prolotherapy may be appropriate for patients who: Have residual instability or discomfort after a shoulder dislocation Show evidence of ligament laxity on physical exam Have failed to achieve stability through physical therapy alone Want to avoid surgery Have generalized ligamentous laxity or hypermobility (e.g., Ehlers-Danlos) It is often most effective in those with functional instability (ligament laxity) rather than mechanical instability from severe labral tears

Cell lines The human CRC cell lines, CCK-81 and DLD-1, and the gastric cancer cell line, MKN45, were obtained from the Health Science Research Resources Bank (Osaka, Japan)

[DOI] [PubMed] [Google Scholar] 41.Hernndez-Hernndez C., Viera-Alcaide I., Morales-Sillero A.M., Fernndez-Bolaos J., Rodrguez-Gutirrez G

e , Relative abundance of SLC25A39, GCLM and SLC25A40 transcripts in HEK-293T cells treated with BSO (1 mM) for the indicated days using quantitative reverse transcription PCR (RT-qPCR), normalized to transcripts of -ACTIN