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The authors reported that the compound restored NAD+ levels in the hearts of the test animals to those of normal mice

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[PMC free article: PMC2810796] [PubMed: 20002079] 89

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The cells were then washed using MACS buffer (0.5% BSA + 2mM EDTA in PBS) followed by staining with different clones of anti-CD38: LS198-4-2 (Beckman Coulter), JK36 (Beckman Coulter) and HIT2 (BD Biosciences) for 10 mins in the dark at room temperature

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