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For the IFN-I bioassay, 5 x 10 4 3T3 cells containing an interferon-sensitive response element (ISRE) fused to luciferase 51 were plated per well into 96-well white-bottom plates (Greneir 655083) in DMEM containing 10% FBS, 100 U/ml penicillin and 100 g/ml streptomycin

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In contrast, the size of the CUR/miR155@DD-Hb NPs exhibited a significant increase of ~ 20 nm during the same time frame

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A key parameter in this analysis is the second-order perturbation energy E 2 , which indicates the strength of orbital interactions (higher E 2 values suggest a more efficient sensor with better drug-binding ability)

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