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Once the supernatant harvested, the nuclear pellets were resuspended in a lysis buffer without any detergent (50 mM Tris, pH 7.5, 150 mM NaCl, 1 mM EDTA) and three cycles of freeze-thaw performed to break the nuclei and release the nuclear matrix

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A comparison of the efficacy of silicone gel containing onion extract and aloe vera to silicone gel sheets to prevent postoperative hypertrophic scars and keloids