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The peptides obtained with the FASP protein digestion were analyzed on a nanoflow liquid chromatography (nLC) system (Ultimate 3000 UHPLC) coupled to an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific, Waltham, MA, United States), operating in positive ionization mode with spray voltage set at 1.7 kV and source temperature at 275 C, equipped with a nanoEASY-Spray ion source

Brni, Carotenoid content and profiles of pumpkin products and by-products

Injectable forms provide higher bioavailability and more consistent results, while oral forms offer convenience but reduced efficiency

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