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These are the challenges in diagnosing fulminant WD which requires time pressure because only urgent transplantation, requiring a definite diagnosis, can save the lives of the patients (22, 30)

mouse monoclonal anti-PrP SAF32 (Bertin Bioreagent, A03202, dilution: 1: 50 for IF), rabbit anti-Myc tag (Millipore, 06549, dilution 1:100), mouse monoclonal anti-K/Na-ATPase (Abcam, ab7671, dilution 1:100), mouse anti-GAPDH (Santa Cruz Biotechnology, sc-32233, dilution 1:1,000 for WB), rabbit monoclonal anti-ATP7B (Abcam, Ab 124973, dilution 1:1,000 for WB) or mouse monoclonal anti-Vinculin (SigmaAldrich, V9264, dilution 1:5,000 for WB)