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after 3 months of treatment, notable improvement was noted on right side in the form of decreased shades of pigmentary network (in cases of epidermal melasma) and decreased irregularity of pigmentary network, decreased number of granules and globules (in cases of mixed melasma)

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Minimal inhibitory concentration (MIC) and minimal algaecidal concentration (MAC) determination In the absence of universally accepted procedures or interpretative criteria specific for Prototheca spp., determination of MICs of 3BP and AMB was performed according to the broth microdilution method, in 96-well microtiter plates (NuncTM, Denmark), strictly following the Clinical Laboratory Standards Institute (CLSI) protocol (M27-A3) for drug susceptibility testing of yeast-like fungi (CLSI, in vitro dose-dependent 3BP toxicity against different strains of Prototheca spp

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