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Nayeem, West Virginia University, United States

After PBST washes, membranes were incubated with HRP-conjugated secondary antibodies (Proteintech, 1:10,000) for 1 h at room temperature

(34) found that DSS-treated mice experienced diarrhea, bloody stools, weight loss, increased pro-inflammatory cytokines (IL-6, TNF-, IL-1), and decreased anti-inflammatory cytokines (IL-10) and occludin and claudin-1

Tube formation assay The extracellular matrix gel-induced tube formation assay was used as an in vitro measurement of angiogenesis

[Epub ahead of print] 48

Additionally, our recent work demonstrates in real-time imaging assays that FAs can traffic from LDs to the nucleus to modulate gene expression in brown adipocytes (141)