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The particle-mesh Ewald method 60 was employed to calculate long-range electrostatic interactions, whereas a smooth (10-12 ) cutoff was applied to treat van der Waals (vdW) interactions

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In contrast, Zheng et al

Signal of cysteine, which is required for glutathione synthesis (Figure 1E), was selectively depleted within TLS in injured kidneys, whereas it was uniformly distributed in healthy kidneys (Figure 1F)

Cells were detached using 0.25% trypsin-EDTA, collected by centrifugation at a speed of 1000 g for 5 min, and resuspended in PBS

Kwiatkowski, L