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Quantitative real-time PCR (qPCR) was performed using SYBR green master mix and -ACTIN was used as a control

Since liquid biopsy is repeatable with minimal adverse effects, as opposed to radiological assays, which present a cumulative risk, it may be especially beneficial for patients who require long-term surveillance due to genetic cancer predisposition

For instance, a previous study demonstrated a SERS platform based on a functionalized silver-coated PMMA MNA that achieved approximately 93% diagnostic reliability in a diabetic mouse model [107]

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