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Lysates were cleared by centrifugation at 17,000 rpm for 10 min at 4 C

(146) measured the plasma levels of oxidized and reduced cystine and GSH in 1,411 patients undergoing coronary angiography

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Proteins were detected by chemiluminescence using Immobilon Classico or Immobilon Forte (Merck), Lumi-Light substrate (Roche), or SuperSignal West Femto (Thermo Fisher) on a ChemiDoc system (Bio-Rad), and band intensities were quantified with Image Lab/Fiji