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In AggreWell plates (AggreWell 800, Stem Cell Technologies), we scaled up proportionally, taking into account that the AggreWell 800 plate has 300 microwells in each well and used 7,500 portal fibroblasts, 30,000 cholangiocytes and 100 h-HepOrgs (proportion of 1 h-HepOrg to 75 portal fibroblasts and 300 cholangiocytes)

Notably, SRs also participate in intracellular signaling cascades such as the MAPK, NF-B, and JAK-STAT pathwaysmany of which are known to be dysregulated in autoimmune conditions (316, 371373)

Although a detailed description of PPI is beyond the aim of our review, such network partially recapitulates the peculiar crosstalk and close association between the intrinsic biological clock and the intracellular signaling pathways described above, with relevant implications in the therapeutic management of diseases

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At the molecular level, fumonisins inhibit ceramide synthase and disrupt sphingolipid metabolism therefore influence apoptosis and mitosis [109]

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