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Summary Keywords anabolic androgenic steroids, AAS, post-cycle therapy, PCT, falsified medicines, cessation of AAS, SERMs, AIs Citation Blazewicz A, Poplawska M, Daniszewska B, Piorunska K and Karynski M (2025) Illegal and falsified medicines self-administrated in not approved post-cycle therapy after the cessation of anabolic-androgenic steroids qualitative analysis

9 231241 10.1038/nrm2312 [DOI] [PubMed] [Google Scholar] Thathiah A., Horre K., Snellinx A., Vandewyer E., Huang Y., Ciesielska M., et al

Add the clear sample containing target GST-tagged protein onto the equilibrated gravity column, keep the sample in column for at least 2 min, ensure that the sample fully binds with the resin, and collect the flowthrough with a flow-rate of 0.5 - 1 mL per minute, then add the flowthrough onto the column again, repeat the previous operation, this can increase the binding efficiency

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