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Effects of fenugreek, garden cress, and black seed on theophylline pharmacokinetics in beagle dogs

For cloning, the amplified products were purified using QIAquick PCR Purification kit (Qiagen Inc) and incubated at 72 C for 10 min in a reaction mixture (25 L) containing 200 M of dATP, 1.5 mM MgCl 2 , 0.625 unit of Taq DNA polymerase and 1X buffer, to add A-tail

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National survey of Japanese patients with mevalonate kinase deficiency reveals distinctive genetic and clinical characteristics