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Liu Y et al (2018) Pharmacological investigation of the Anti-Inflammation and Anti-Oxidation activities of Diallyl disulfide in a rat emphysema model induced by cigarette smoke extract

After 24 h, the medium was replaced with 5 M dye (1 mL) in phenol red-free medium followed by incubation for 15 min, and images were acquired at200 magnification under a fluorescence microscope (Leica Microsystems, Wetzlar, Germany)