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The NEJM article discusses people who had strong reactions to dose 1 and what they did to counter them

reactions persisting beyond 48 hours or involving induration suggest sensitivity to the bacteriostatic water preservative (benzyl alcohol) rather than the peptide itself

The specific day does not matter, but the consistency does

Unfortunately, no

SynergyFinder 3.0: an interactive analysis and consensus interpretation of multi-drug synergies across multiple samples

Some of the characteristic symptoms of this deficiency include: extreme tiredness lack of energy breathlessness feeling faint headaches pale skin noticeable heartbeats (palpitations) loss of appetite and weight loss a decline in your mental abilities (brain fog) irritability depression If youre following a vegan diet and recognise any of the symptoms outlined above (or just wish to prevent them!), a vegan B12 supplement may be required and you have two options available