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Sucher NJ, Wong LA, Lipton SA
Abbreviations Ac-DEVD-pNA: Caspase-3 colorimetric substrate AhR: Aryl hydrocarbon receptor ARNT: AhR nuclear translocator BBB: Blood-brain barrier BDNF: Brain-derived neurotrophic factor BGM: Brain-gut-microbiome BSA: Bovine serum albumin BV-2: Microglial cell line CAY10464: 1,3-dichloro-5-[(1E)-2-(4-methoxyphenyl)ethenyl]-benzene CHAPS: 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate CHOP: C/EBP homologous protein CNS: Central nervous system COX-2: Cyclooxygenase-2 DMEM: Dulbeccos Modified Eagle Medium DRE: Dioxin-responsive element DTT: DL-dithiothreitol EDTA: Ethylenediaminetetraacetic acid ERK1/2: Extracellular signal-regulated kinase-1/2 FBS: Fetal bovine serum GSH: Glutathione HPLC-ESI-QTOF-MS/MS : High-performance liquid chromatographyelectrospray ionizationquadrupole time-of-flighttandem mass spectrometry HPMEC: Human fetal pulmonary microvascular endothelial cells HSP90 : Heat shock protein 90 HT-22 : Mouse hippocampal cell line IL : Interleukin iNOS : nducible nitric oxide synthase INT: Iodonitrotetrazolium salt LDH: Lactate dehydrogenase MAPK: Mitogen-activated protein kinases M-CFS: Microbiome cell-free supernatant MPMS: 1-methoxy-5-methylphenazinium methyl sulfate NaCl: Sodium chloride NAD: -nicotinamide adenine dinucleotide NFTs: Neurofibrillary tangles NGF: Nerve growth factor NO: Nitric oxide NIH-3T3: Mouse fibroblast cell line PBS: Phosphate-buffer saline PCNA: Proliferating cell nuclear antigen PI: Propidium iodide RAW 264.7: Murine macrophage cell line ROS: Reactive oxygen species SOD1: Superoxide dismutase-1 SRC: Proto-oncogene tyrosine- kinase TEMED: N,N,N,N-tetramethyl ethylenediamine TNF-: Tumor necrosis factor alpha SHIME: Simulator of Human Microbial Intestinal Ecosystem References Tremlett H, Bauer KC, Appel-Cresswell S, Finlay BB, Waubant E
Fleseriu M, Skugor M, Chinnappa P, Siraj ES
The platform delivered IL-4 into inflammatory tissues, achieving M1-to-M2 polarization in LPS-stimulated BMDMs and mice colon tissue in dextran sulfate sodium salt-induced acute colitis, thus protecting against IBD
