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RNA was reverse transcribed in a final volume of 20 l containing 1 RT buffer (500 mM each dNTP, 3 mM MgCl2, 75 mM KCl, 50 mM Tris-HCl pH 8.3), 10 units of RNasinTM ribonuclease inhibitor (Promega, Madison, #N2511, WI, USA), 10 mM dithiothreitol, 50 units of Superscript II RNase H- reverse transcriptase (Gibco-BRL, #18064014, Gaithersburg, MD, USA), 1.5 mM random hexamers (Pharmacia, Uppsala, Sweden) and 1 g of total RNA

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Alternatively, a number of clinical trials have been conducted in the recent past to examine the radiomodulating effect of selenium in general for improving the quality of life of cancer patients undergoing radiotherapy [95,96,97,98,99]