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After a second centrifugation, the pellet was resuspended in an equal volume of PBS and 2 loading buffer and boiled for 5 min before immunoblotting

Results from one of three independent experiments are shown

doi: 10.1111/nyas.13031 129

Increasing or decreasing in the external osmolarity beyond normal range, termed hypertonic or hypotonic stress, respectively, triggers water fluxes across semipermeable cytoplasmic membrane

Neuzillet, C

Because dietary vitamin E is essential to prevent vitamin E deficiency in newborns, vitamin E must be supplied in parenteral nutrition solutions in infants who cannot be given enteral feeding, such as prematurely born infants